Reference SummaryWilliams JL, Biochem Biophys Res Commun 2004 Jan 16;313(3):784-8

Title

 NO-donating aspirin inhibits intestinal carcinogenesis in Min (APC(Min/+)) mice.

Authors

 Williams JL; Kashfi K; Ouyang N; del Soldato P; Kopelovich L; Rigas B

Journal

 Biochem Biophys Res Commun

Volume

 313

Issue

 3

Year

 2004

Pages

 784-8

Abstract

 The chemopreventive effect of nitric oxide-releasing aspirin (NO-ASA) against gastrointestinal tumorigenesis was evaluated in Min (APC(Min/+)) mice. NO-ASA consists of a traditional ASA that bears covalently attached to it an NO-releasing moiety. Four groups (N=10) of six-week-old female C57BL/6J APC(Min/+) and the corresponding C57BL/6J(+/+) wild type mice were treated either with vehicle or NO-ASA 100 mg/kg/day intrarectally for 21 days. There were no signs of overt toxicity including gastrointestinal toxicity from NO-ASA. Vehicle treated Min mice had 24.7 +/- 3.8 tumors (mean +/- SEM) and NO-ASA treated Min mice had 10.1 +/- 1.4 tumors (59% reduction; P<0.001). Wild type mice showed no tumors. NO-ASA did not affect cell proliferation in small intestinal mucosa, determined by immunohistochemical staining for PCNA. Our findings establish the strong inhibitory effect of NO-ASA in intestinal carcinogenesis in the Min mouse and suggest that this agent merits further evaluation as a chemopreventive agent against colon cancer.

Links

 J:87459 – MGI References
14697260 – National Library of Medicine/PubMed

Strain Notes

Strain Note
C57BL/6J Treatment with NO-ASA had no apparent effect on body weight.
Wild type mice on C57BL/6J background. Littermate controls for ApcMin/+ mice, purchased from The Jackson Laboratory (TJL Stock Number 2020).
C57BL/6J-ApcMin/+ 6 week old mice were purchased from The Jackson Laboratory.
Treatment with NO-ASA had no apparent effect on body weight.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J-ApcMin/+ Intestine - Small Intestine tumor Intestine - Small Intestine

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine tumor
  • 2-(acetyloxy)benzoic acid 4-(nitrooxy methyl)phenyl ester) (NO-ASA)
 Intestine - Small Intestine

90
C57BL/6J Intestine - Small Intestine tumor Intestine - Small Intestine

0
C57BL/6J Intestine - Small Intestine tumor
  • 2-(acetyloxy)benzoic acid 4-(nitrooxy methyl)phenyl ester) (NO-ASA)
 Intestine - Small Intestine

0