Reference SummaryBashir O, Carcinogenesis 2004 Aug;25(8):1507-15

Title

Both suboptimal and elevated vitamin intake increase intestinal neoplasia and alter crypt fission in the ApcMin/+ mouse.

Authors

Bashir O; FitzGerald AJ; Goodlad RA

Journal

Carcinogenesis

Volume

25

Issue

8

Year

2004

Pages

1507-15

Abstract

The effects of vitamin deficiency on intestinal cancer are unclear, and even less is known about the consequences of excessive intake. We therefore investigated the actions of altered vitamin content on intestinal polyp development, cell proliferation and crypt fission in a mouse model of neoplasia. Ninety multiple intestinal neoplasia (Apc(Min/+)) mice and 90 wild-type littermates, 4 weeks old, were divided into six groups and fed either a control semi-synthetic diet, or the semi-synthetic diet with the vitamin content lowered to a third of the RDA or the semi-synthetic diet with the vitamin content increased 5-fold (except for retinol and folate, which were doubled). The number and size of polyps in the small and large intestines were scored after 8 weeks on the diets, as was cell proliferation (native mitoses per crypt) and crypt fission. The small intestines of the low and high vitamin groups were heavier than the controls. There were significantly more polyps and the tumour burden was higher in both the low and the high vitamin groups (P < 0.02). Proliferation was slightly reduced by the vitamin alteration and crypt fission was significantly increased in the Apc(Min/+) mice when compared with the wild-type (P < 0.001). Fission indices were decreased by vitamin alteration in the small intestine, and increased in the colon, but only in the Apc(Min/+) mice. The effects of vitamin alteration on polyp number were most pronounced in the proximal intestine, which is also the site of maximum crypt fission. Both vitamin deficiency and over-supplementation can markedly enhance polyp number and tumour burden.

Links

J:91525 – Mouse Genome Informatics
15016659 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J Intestine - Large Intestine - Colon polyp Intestine - Large Intestine - Colon

0

C57BL/6J Intestine - Large Intestine - Colon polyp
  • low vitamin
Intestine - Large Intestine - Colon

0

C57BL/6J Intestine - Large Intestine - Colon polyp
  • high vitamin
Intestine - Large Intestine - Colon

0

C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp Intestine - Large Intestine - Colon

observed

C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp
  • low vitamin
Intestine - Large Intestine - Colon

observed

C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp
  • high vitamin
Intestine - Large Intestine - Colon

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp Intestine - Small Intestine - Distal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp
  • low vitamin
Intestine - Small Intestine - Distal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp
  • high vitamin
Intestine - Small Intestine - Distal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp Intestine - Small Intestine - Medial

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp
  • low vitamin
Intestine - Small Intestine - Medial

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp
  • high vitamin
Intestine - Small Intestine - Medial

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp Intestine - Small Intestine - Proximal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp
  • low vitamin
Intestine - Small Intestine - Proximal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp
  • high vitamin
Intestine - Small Intestine - Proximal

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp Intestine - Small Intestine

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp
  • low vitamin
Intestine - Small Intestine

observed

C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp
  • high vitamin
Intestine - Small Intestine

observed

C57BL/6J Intestine - Small Intestine polyp Intestine - Small Intestine

0

C57BL/6J Intestine - Small Intestine polyp
  • low vitamin
Intestine - Small Intestine

0

C57BL/6J Intestine - Small Intestine polyp
  • high vitamin
Intestine - Small Intestine

0