Reference SummaryKlein IK, Cancer Res 2000 Apr 15;60(8):2077-80

Title

Adenoma-specific alterations of protein kinase C isozyme expression in Apc(MIN) mice

Authors

Klein IK; Ritland SR; Burgart LJ; Ziesmer SC; Roche PC; Gendler SJ; Karnes WE Jr

Journal

Cancer Res

Volume

60

Issue

8

Year

2000

Pages

2077-80

Abstract

Members of the protein kinase C (PKC) family appear to play important roles in colorectal carcinogenesis. To investigate the potential involvement of PKC isozymes in adenomatous transformation induced by inactivation of the adenomatous polyposis coli (APC) gene product, we examined protein levels and localizations of ten PKC isozymes by immunohistochemistry in normal and adenomatous ileal epithelium of ApcMIN mice. Compared with surrounding normal epithelium, adenomas showed dramatically reduced staining for PKCs a, beta1, and zeta, as well as dysplasia-specific punctate nuclear staining of PKC mu. We conclude that reduced protein expression of PKC alpha, beta1, and zeta, and nuclear localization of PKC mu are markers of, and are perhaps involved in, adenomatous transformation induced by APC inactivation in ApcMIN mice.

Links

J:61731 – Mouse Genome Informatics
10786662 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Ileum adenoma Intestine - Small Intestine - Ileum

observed