Reference SummarySchonhuber N, Nat Med 2014 Nov;20(11):1340-7

Title

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.

Authors

Schonhuber N; Seidler B; Schuck K; Veltkamp C; Schachtler C; Zukowska M; Eser S; Feyerabend TB; Paul MC; Eser P; Klein S; Lowy AM; Banerjee R; Yang F; Lee CL; Moding EJ; Kirsch DG; Scheideler A; Alessi DR; Varela I; Bradley A; Kind A; Schnieke AE; Rodewald HR; Rad R; Schmid RM; Schneider G; Saur D

Journal

Nat Med

Volume

20

Issue

11

Year

2014

Pages

1340-7

Abstract

Genetically engineered mouse models (GEMMs) have dramatically improved our understanding of tumor evolution and therapeutic resistance. However, sequential genetic manipulation of gene expression and targeting of the host is almost impossible using conventional Cre-loxP-based models. We have developed an inducible dual-recombinase system by combining flippase-FRT (Flp-FRT) and Cre-loxP recombination technologies to improve GEMMs of pancreatic cancer. This enables investigation of multistep carcinogenesis, genetic manipulation of tumor subpopulations (such as cancer stem cells), selective targeting of the tumor microenvironment and genetic validation of therapeutic targets in autochthonous tumors on a genome-wide scale. As a proof of concept, we performed tumor cell-autonomous and nonautonomous targeting, recapitulated hallmarks of human multistep carcinogenesis, validated genetic therapy by 3-phosphoinositide-dependent protein kinase inactivation as well as cancer cell depletion and show that mast cells in the tumor microenvironment, which had been thought to be key oncogenic players, are dispensable for tumor formation.

Links

J:218155 – Mouse Genome Informatics
25326799 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Intestine - Small Intestine - Duodenum adenoma Intestine - Small Intestine - Duodenum

7

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Intestine - Small Intestine - Duodenum adenoma - serrated Intestine - Small Intestine - Duodenum

very low

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Intestine - Small Intestine - Duodenum hyperplasia Intestine - Small Intestine - Duodenum

100

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Intestine metaplasia Intestine

0

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Liver - Bile duct papilloma Liver - Bile duct

observed - 8

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell metaplasia - ductal Pancreas - Acinar cell

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas - Acinar cell metaplasia - ductal Pancreas - Acinar cell

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell metaplasia - ductal Pancreas - Acinar cell

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas - Acinar cell

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas - Acinar cell

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 1b (PanIN1b) Pancreas - Acinar cell

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 1b (PanIN1b) Pancreas - Acinar cell

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas - Acinar cell

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas - Acinar cell

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 3 (PanIN3) Pancreas - Acinar cell

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Acinar cell pancreatic intraepithelial neoplasia 3 (PanIN3) Pancreas - Acinar cell

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - mixed
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas - Duct adenocarcinoma - moderately differentiated
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - sarcomatoid
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas - Duct adenocarcinoma - undifferentiated
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - undifferentiated
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1.1Dgk/+ Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1.1Dgk Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Tyj/+ Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Tyj Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Trp53tm2Tyj/+ Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Trp53tm2Tyj Pancreas - Duct adenocarcinoma - undifferentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas - Duct adenocarcinoma - well-differentiated
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas - Duct adenocarcinoma - well-differentiated
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1.1Dgk/+ Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1.1Dgk Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Tyj/+ Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Tyj Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Trp53tm2Tyj/+ Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Trp53tm2Tyj Pancreas - Duct adenocarcinoma - well-differentiated Pancreas - Duct

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal Pancreas

observed - very low

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas adenocarcinoma - ductal Pancreas

0

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas adenocarcinoma - ductal
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed - 100

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm2Dym/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1.1Dgk/+ Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Tyj/+ Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Trp53tm2Tyj/+ Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

0

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas adenocarcinoma - ductal Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas lesion Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas lesion
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

0

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas neoplasia
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

very low

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas neoplasia
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN) Pancreas

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas pancreatic intraepithelial neoplasia (PanIN) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Trp53tm1Brn Pancreas pancreatic intraepithelial neoplasia (PanIN) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN) Pancreas

very low

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN) - high grade
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia (PanIN) - low grade
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm1(Tva)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm2Dym/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1a (PanIN1a)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b) Pancreas

0

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b) Pancreas

0

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 1b (PanIN1b)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas

0 - observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas

0 - observed

B6;129S6-Gt(ROSA)26Sortm2Dym/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 2 (PanIN2)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 3 (PanIN3) Pancreas

observed

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Pancreas pancreatic intraepithelial neoplasia 3 (PanIN3) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm2Dym/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 3 (PanIN3) Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 3 (PanIN3)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

0

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas pancreatic intraepithelial neoplasia 3 (PanIN3)
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw Tg(Pdx1-flpo)#Dsa Pancreas tumor Pancreas

0

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Pdpk1tm1.1Mlw/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

100

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

0 - very low

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Cpa3tm3(icre)Hrr/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor Pancreas

observed

B6;129S6-Gt(ROSA)26Sortm1(DTA)Jpmb/+ Gt(ROSA)26Sortm3(CAG-Cre/ERT2)Dsa/+ Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor - nodular
  • tamoxifen (For inducing expression from mutant alleles)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Pancreas tumor - nodular
  • tamoxifen (TAM)
Pancreas

observed

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa Skin papilloma Skin

observed - 8

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv Skin papilloma Skin

80

B6;129S6-Krastm1Dsa/+ Tg(Pdx1-flpo)#Dsa (Unspecified organ) carcinoma (Unspecified organ)

0

B6;129S6-Krastm4Tyj/+ Tg(Pdx1-cre)6Tuv (Unspecified organ) carcinoma (Unspecified organ)

0