Reference SummaryRyan J, J Natl Cancer Inst 1987 Dec;79(6):1351-7

Title

KRAS2 as a genetic marker for lung tumor susceptibility in inbred mice.

Authors

Ryan J; Barker PE; Nesbitt MN; Ruddle FH

Journal

J Natl Cancer Inst

Volume

79

Issue

6

Year

1987

Pages

1351-7

Abstract

An Eco-RI restriction fragment length polymorphism occurring in a DNA fragment containing the first exon of the murine KRAS2 gene was shown to correlate with the inherited susceptibility of inbred strains of mice to urethan (CAS: 51-79-6)-induced pulmonary adenomas. Eco-RI digestion of murine DNA yielded four KRAS2-specific fragments. Polymorphic variation occurred in the smallest molecular-weight fragment with alleles of either 0.70 or 0.55 kb in size. Genotyping of 14 inbred strains of mice revealed a correlation between KRAS2 Eco-RI polymorphic variation and the differential susceptibility among inbred strains to development of pulmonary adenomas. Strains with a high incidence of pulmonary adenomas, either spontaneously occurring or in response to carcinogen induction, had the 0.55-kb KRAS2 allele whereas adenoma-resistant strains had the 0.70-kb allele. Analysis of a series of recombinant inbred strains (AXB, BXA) that developed from reciprocal crosses between a highly susceptible strain (A/J) and a highly resistant strain (C57BL/6J) revealed a statistically significant threefold difference in lung tumor susceptibility on the basis of KRAS2 genotype. Further analysis of individual F2 mice of a C57BL/6 female X A/J male cross also demonstrated a threefold difference in tumor susceptibility on the basis of KRAS2 allelic variation.

Links

J:17778 – Mouse Genome Informatics
2891865 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
AXB1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB2 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB3 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB4 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB5 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB6 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB7 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB8 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB9 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB10 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB11 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB12 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB13 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB14 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB15 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB17 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB19 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB20 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB21 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB22 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB23 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

AXB24 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA2 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA4 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA8 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA9 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA10 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA12 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA13 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA14 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA16 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA17 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA18 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA19 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA20 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA22 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA23 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA24 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

BXA25 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

(C57BL/6J x A/J)F2 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

C57BL/6J x B6AF1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

observed

A/J Lung tumor Lung

90

A/HeJ Lung tumor Lung

90

SWR/J Lung tumor Lung

90

BALB/cJ Lung tumor Lung

40.0 - 50.0

CBA/J Lung tumor Lung

20

RF/J Lung tumor Lung

40

C3H/HeJ Lung tumor Lung

5.0 - 10.0

C57L/J Lung tumor Lung

1

C57BL/6J Lung tumor Lung

1

DBA/2J Lung tumor Lung

5.0 - 10.0

SJL/J Lung tumor Lung

5

AKR/J Lung tumor Lung

5