Reference SummaryUeda T, Proc Natl Acad Sci U S A 2010 Aug 10;107(32):13984-90

Title

Combined deficiency for MAP kinase-interacting kinase 1 and 2 (Mnk1 and Mnk2) delays tumor development.

Authors

Ueda T; Sasaki M; Elia AJ; Chio II; Hamada K; Fukunaga R; Mak TW

Journal

Proc Natl Acad Sci U S A

Volume

107

Issue

32

Year

2010

Pages

13984-90

Abstract

MAP kinase-interacting kinase 1 and 2 (Mnk1 and Mnk2) are protein-serine/threonine kinases that are activated by ERK or p38 and phosphorylate eIF4E, which is involved in cap-dependent translation initiation. However, Mnk1/2 double knockout (Mnk-DKO) mice show normal cell growth and development despite an absence of eIF4E phosphorylation. Here we show that the tumorigenesis occurring in the Lck-Pten mouse model (referred to here as tPten(-/-) mice) can be suppressed by the loss of Mnk1/2. Phosphorylation of eIF4E was greatly enhanced in lymphomas of parental tPten(-/-) mice compared with lymphoid tissues of wild-type mice, but was totally absent in lymphomas of tPten(-/-); Mnk-DKO mice. Notably, stable knockdown of Mnk1 in the human glioma cell line U87MG resulted in dramatically decreased tumor formation when these cells were injected into athymic nude mice. Our data demonstrate an oncogenic role for Mnk1/2 in tumor development, and highlight these molecules as potential anticancer drug targets that could be inactivated with minimal side effects.

Links

J:163707 – Mouse Genome Informatics
20679220 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6-Mknk1tm1Fuku/Mknk1? Mknk2tm1Fuku/Mknk1? Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Leukocyte - Lymphocyte - T-lymphocyte

11 - 73

C57BL/6-Mknk1tm1Fuku Mknk2tm1Fuku Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Leukocyte - Lymphocyte - T-lymphocyte

100

C57BL/6-Mknk1tm1Fuku/+ Mknk2tm1Fuku/+ Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Spleen

observed

C57BL/6-Mknk1tm1Fuku/+ Mknk2tm1Fuku/+ Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Thymus

observed

C57BL/6-Mknk1tm1Fuku Mknk2tm1Fuku Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Spleen

observed

C57BL/6-Mknk1tm1Fuku Mknk2tm1Fuku Ptentm2Mak Tg(Lck-cre)548Jxm Leukocyte - Lymphocyte - T-lymphocyte lymphoma Thymus

observed